Description: Recombinant rabbit monoclonal antibody to KIF5B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human KIF5B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: KIF5B Alternative Names: KNS; KNS1; Kinesin-1 heavy chain; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHCEntrez Gene (Human): 3799SwissProt (Human): P33176Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of KIF5B expression in HEK293T (A), K562 (B), MCF7 (C) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 120 kD)
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  Immunohistochemical analysis of KIF5B staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).