Description: Recombinant rabbit monoclonal antibody to GM130 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human GM130. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GOLGA2 Alternative Names: Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; Golgin-95Entrez Gene (Human): 2801SwissProt (Human): Q08379Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GM130 expression in HEK293T (A), K562 (B), Hela (C) whole cell lysates. (Predicted band size: 113 kD; Observed band size: 130 kD)
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  Immunohistochemical analysis of GM130 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).