Description: Recombinant rabbit monoclonal antibody to NUP50 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human NUP50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NUP50 Alternative Names: NPAP60L; Nuclear pore complex protein Nup50; 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50Entrez Gene (Human): 10762SwissProt (Human): Q9UKX7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NUP50 expression in HEK293T (A), Jurkat (B), THP1 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD)
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  Immunohistochemical analysis of NUP50 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).