Description: Recombinant rabbit monoclonal antibody to ACAT2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ACAT2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ACAT2 Alternative Names: ACTL; Acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolaseEntrez Gene (Human): 39Entrez Gene (Mouse) : 110460Entrez Gene (Rat) : 308100SwissProt (Human): Q9BWD1SwissProt (Mouse) : Q8CAY6SwissProt (Rat) : Q5XI22Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ACAT2 expression in HEK293T (A), HepG2 (B), K562 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 38 kD)
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Immunohistochemical analysis of ACAT2 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).