Description: Recombinant rabbit monoclonal antibody to ACAA2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ACAA2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ACAA2 Alternative Names: 3-ketoacyl-CoA thiolase, mitochondrial; Acetyl-CoA acyltransferase; Beta-ketothiolase; Mitochondrial 3-oxoacyl-CoA thiolase; T1Entrez Gene (Human): 10449Entrez Gene (Mouse) : 52538Entrez Gene (Rat) : 170465SwissProt (Human): P42765SwissProt (Mouse) : Q8BWT1SwissProt (Rat) : P13437Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ACAA2 expression in HEK293T (A), HepG2 (B), Hela (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 45 kD)
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  Immunohistochemical analysis of ACAA2 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).