Description: Recombinant rabbit monoclonal antibody to PKN2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human PKN2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: PKN2 Alternative Names: PRK2; PRKCL2; Serine/threonine-protein kinase N2; PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2Entrez Gene (Human): 5586SwissProt (Human): Q16513Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKN2 expression in HEK293T (A), HepG2 (B), Hela (C) whole cell lysates. (Predicted band size: 112 kD; Observed band size: 112 kD)
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  Immunohistochemical analysis of PKN2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PKN2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).