Description: Recombinant rabbit monoclonal antibody to Beta-catenin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Beta-catenin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CTNNB1 Alternative Names: CTNNB; Catenin beta-1; Beta-cateninEntrez Gene (Human): 1499Entrez Gene (Mouse) : 12387SwissProt (Human): P35222SwissProt (Mouse) : Q02248Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta-catenin expression in HEK293T (A), H1792 (B), HCT116 (C), mouse liver (D) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 92 kD)
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Immunohistochemical analysis of Beta-catenin staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).