Recombinant Anti-WNT2B Rabbit mAb-Primary Antibody-Cohesion Biosciences

Western blot analysis of WNT2B expression in HEK293T (A), H1792 (B), HCT116 (C) whole cell lysates. (Predicted band size: 43; 41 kD; Observed band size: 44 kD)
Immunofluorescent analysis of WNT2B staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name	Recombinant Anti-WNT2B Rabbit mAb
Cat No:	CMA4202
Source:	Rabbit
Reactivity:	H
Applications:	WB, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Recombinant rabbit monoclonal antibody to WNT2B

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human WNT2B. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Gene Symbol: WNT2B

Alternative Names: WNT13; Protein Wnt-2b; Protein Wnt-13

Entrez Gene (Human): 7482

SwissProt (Human): Q93097

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of WNT2B expression in HEK293T (A), H1792 (B), HCT116 (C) whole cell lysates. (Predicted band size: 43; 41 kD; Observed band size: 44 kD)
Immunofluorescent analysis of WNT2B staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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