Description: Recombinant rabbit monoclonal antibody to SEC61A2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human SEC61A2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: SEC61A2 Alternative Names: Protein transport protein Sec61 subunit alpha isoform 2; Sec61 alpha-2Entrez Gene (Human): 55176Entrez Gene (Mouse) : 57743SwissProt (Human): Q9H9S3SwissProt (Mouse) : Q9JLR1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SEC61A2 expression in HEK293T (A), A549 (B), THP1 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 48 kD)
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  Immunofluorescent analysis of SEC61A2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).