Description: Recombinant rabbit monoclonal antibody to BAX Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human BAX. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: BAX Alternative Names: BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4Entrez Gene (Human): 581Entrez Gene (Mouse) : 12028SwissProt (Human): Q07812SwissProt (Mouse) : Q07813Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BAX expression in HEK293T (A), A549 (B), THP1 (C), mouse liver (D), mouse kidney (E) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD)
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Immunohistochemical analysis of BAX staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).