Description: Recombinant rabbit monoclonal antibody to EOGT Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human EOGT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: EOGT Alternative Names: AER61; C3orf64; EOGT1; EGF domain-specific O-linked N-acetylglucosamine transferase; Extracellular O-linked N-acetylglucosamine transferaseEntrez Gene (Human): 285203Entrez Gene (Mouse) : 101351SwissProt (Human): Q5NDL2SwissProt (Mouse) : Q8BYW9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of EOGT expression in HEK293T (A), A549 (B), Hela (C), mouse kidney (D) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 55 kD)
-
Immunofluorescent analysis of EOGT staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).