Description: Recombinant rabbit monoclonal antibody to OTX1/2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human OTX1/2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: OTX1; OTX2 Alternative Names: Homeobox protein OTX1; Orthodenticle homolog 1; Homeobox protein OTX2; Orthodenticle homolog 2Entrez Gene (Human): 5013; 5015SwissProt (Human): P32242; P32243Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of OTX1/2 expression in HEK293T (A) whole cell lysates. (Predicted band size: 37; 31; 32 kD; Observed band size: 32 kD)
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Immunohistochemical analysis of OTX1/2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).