Description: Recombinant rabbit monoclonal antibody to Cadherin-pan Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cadherin-pan. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: CDH1; CDH2; CDH3; CDH4; CDH5 Alternative Names: CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD325; CDHP; Cadherin-3; Placental cadherin; P-cadherin; Cadherin-4; Retinal cadherin; R-CAD; R-cadherin; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD144Entrez Gene (Human): 999; 1000; 1001; 1002SwissProt (Human): P12830; P19022; P22223; P55283; P33151Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cadherin-pan expression in HEK293T (A) whole cell lysates. (Predicted band size: 97; 90 kD; Observed band size: 140 kD)
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  Immunofluorescent analysis of Cadherin-pan staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).