Description: Recombinant rabbit monoclonal antibody to Histone H2A (Acetyl-K9) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K9 of human Histone H2A protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: HIST1H2AB; HIST1H2AE Alternative Names: H2AFM; H2AFA; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/mEntrez Gene (Human): 3012; 8335SwissProt (Human): P04908Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H2A (Acetyl-K9) expression in H1762 (A), LO2 (B) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14 kD)
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  Immunofluorescent analysis of Histone H2A (Acetyl-K9) staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).