Description: Recombinant rabbit monoclonal antibody to HSP27 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human HSP27. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: HSPB1 Alternative Names: HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27Entrez Gene (Human): 3315Entrez Gene (Mouse) : 15507Entrez Gene (Rat) : 24471SwissProt (Human): P04792SwissProt (Mouse) : P14602SwissProt (Rat) : P42930Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSP27 expression in A549 (A), THP1 (B), PC3 (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)