Description: Recombinant rabbit monoclonal antibody to UBC9 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human UBC9. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: UBE2I Alternative Names: UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18Entrez Gene (Human): 7329Entrez Gene (Mouse) : 102641751; 22196Entrez Gene (Rat) : 25573SwissProt (Human): P63279SwissProt (Mouse) : P63280SwissProt (Rat) : P63281Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBC9 expression in A549 (A), THP1 (B), K562 (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)
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  Immunofluorescent analysis of UBC9 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).