Recombinant Anti-DHFR Rabbit mAb-Primary Antibody-Cohesion Biosciences

Western blot analysis of DHFR expression in A549 (A), Hela (B), THP1 (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD)
Immunofluorescent analysis of DHFR staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name	Recombinant Anti-DHFR Rabbit mAb
Cat No:	CMA4098
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Recombinant rabbit monoclonal antibody to DHFR

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human DHFR. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Gene Symbol: DHFR

Alternative Names: Dihydrofolate reductase

Entrez Gene (Human): 1719

Entrez Gene (Mouse) : 13361

Entrez Gene (Rat) : 24312

SwissProt (Human): P00374

SwissProt (Mouse) : P00375

SwissProt (Rat) : Q920D2

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of DHFR expression in A549 (A), Hela (B), THP1 (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD)
Immunofluorescent analysis of DHFR staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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