Description: Recombinant rabbit monoclonal antibody to ACTL8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ACTL8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: ACTN4 Alternative Names: Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4Entrez Gene (Human): 81Entrez Gene (Mouse) : 60595Entrez Gene (Rat) : 63836SwissProt (Human): O43707SwissProt (Mouse) : P57780SwissProt (Rat) : Q9QXQ0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ACTL8 expression in A549 (A), Hela (B), HepG2 (C), mouse liver (D), rat liver (E) whole cell lysates. (Predicted band size: 104 kD; Observed band size: 105 kD)
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Immunofluorescent analysis of ACTL8 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).