Description: Recombinant rabbit monoclonal antibody to ASH2L Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ASH2L. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ASH2L Alternative Names: ASH2L1; Set1/Ash2 histone methyltransferase complex subunit ASH2; ASH2-like proteinEntrez Gene (Human): 9070SwissProt (Human): Q9UBL3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ASH2L expression in HEK293T (A), THP1 (B), Jurkat (C) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 69; 85 kD)
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Immunohistochemical analysis of ASH2L staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).