Description: Recombinant rabbit monoclonal antibody to SHH Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human SHH. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SHH Alternative Names: Sonic hedgehog protein; SHH; HHG-1Entrez Gene (Human): 6469Entrez Gene (Mouse) : 20423SwissProt (Human): Q15465SwissProt (Mouse) : Q62226Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SHH expression in EC9706 (A), mouse liver (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 51 kD)
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  Immunohistochemical analysis of SHH staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).