Description: Recombinant rabbit monoclonal antibody to ACC alpha/beta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ACC alpha/beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ACACA; ACACB Alternative Names: ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha; ACC2; ACCB; Acetyl-CoA carboxylase 2; ACC-betaEntrez Gene (Human): 31; 32Entrez Gene (Mouse) : 107476SwissProt (Human): Q13085; O00763SwissProt (Mouse) : Q5SWU9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ACC alpha/beta expression in mouse liver (A) whole cell lysates. (Predicted band size: 265 kD; Observed band size: 265 kD)
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Immunohistochemical analysis of ACC alpha/beta staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).