Description: Recombinant rabbit monoclonal antibody to AK2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human AK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: AK2 Alternative Names: ADK2; Adenylate kinase 2, mitochondrial; AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinaseEntrez Gene (Human): 204Entrez Gene (Mouse) : 11637Entrez Gene (Rat) : 24184SwissProt (Human): P54819SwissProt (Mouse) : Q9WTP6SwissProt (Rat) : P29410Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AK2 expression in HEK293T (A), MCF7 (B), mouse lung (C), mouse kidney (D), rat lung (E), rat kidney (F) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 26 kD)
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  Immunofluorescent analysis of AK2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).