Description: Recombinant rabbit monoclonal antibody to AK4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human AK4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: AK4 Alternative Names: AK3; AK3L1; Adenylate kinase 4, mitochondrial; AK 4; Adenylate kinase 3-like; GTP:AMP phosphotransferase AK4Entrez Gene (Human): 100507855; 205SwissProt (Human): P27144Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AK4 expression in HEK293T (A), MCF7 (B), Jurkat (C) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)
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  Immunofluorescent analysis of AK4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).