Description: Recombinant rabbit monoclonal antibody to MGST1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human MGST1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MGST1 Alternative Names: GST12; MGST; Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-IEntrez Gene (Human): 4257Entrez Gene (Mouse) : 56615Entrez Gene (Rat) : 171341SwissProt (Human): P10620SwissProt (Mouse) : Q91VS7SwissProt (Rat) : P08011Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MGST1 expression in mouse live (A), rat liver (B) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 15 kD)
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Immunohistochemical analysis of MGST1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).