Description: Recombinant rabbit monoclonal antibody to HPRT Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human HPRT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HPRT1 Alternative Names: HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTaseEntrez Gene (Human): 3251Entrez Gene (Mouse) : 15452Entrez Gene (Rat) : 24465SwissProt (Human): P00492SwissProt (Mouse) : P00493SwissProt (Rat) : P27605Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HPRT expression in HEK293T (A), THP1 (B), HCT116 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
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Immunohistochemical analysis of HPRT staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).