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Recombinant Anti-HPRT Rabbit mAb CMA4014
  • Western blot analysis of HPRT expression in HEK293T (A), THP1 (B), HCT116 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
  • Immunohistochemical analysis of HPRT staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Product NameRecombinant Anti-HPRT Rabbit mAb
Cat No: CMA4014
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Recombinant rabbit monoclonal antibody to HPRT
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human HPRT. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Monoclonal
Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol: HPRT1
Alternative Names: HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase
Entrez Gene (Human): 3251
Entrez Gene (Mouse) : 15452
Entrez Gene (Rat) : 24465
SwissProt (Human): P00492
SwissProt (Mouse) : P00493
SwissProt (Rat) : P27605
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of HPRT expression in HEK293T (A), THP1 (B), HCT116 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
  • Immunohistochemical analysis of HPRT staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
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