Description: Recombinant rabbit monoclonal antibody to Beta-actin Immunogen: Recombinant fusion protein of human Beta-actin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ACTB Alternative Names: Actin, cytoplasmic 1; Beta-actinEntrez Gene (Human): 60Entrez Gene (Mouse) : 11461Entrez Gene (Rat) : 81822SwissProt (Human): P60709SwissProt (Mouse) : P60710SwissProt (Rat) : P60711Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Beta-actin expression in HEK293T (A), H1792 (B), HCT116 (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 45 kD)
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  Immunohistochemical analysis of Beta-actin staining in lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.