Description: Recombinant rabbit monoclonal antibody to BCL2 Immunogen: Recombinant fusion protein of human BCL2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: BCL2 Alternative Names: Apoptosis regulator Bcl-2Entrez Gene (Human): 596Entrez Gene (Mouse) : 12043SwissProt (Human): P10415SwissProt (Mouse) : P10417Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of BCL2 expression in HEK293T (A), Hela (B), Jurkat (C), mouse kidney (D) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 26 kD)
• 
  Immunohistochemical analysis of BCL2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.