Description: Recombinant rabbit monoclonal antibody to CD8a Immunogen: Recombinant fusion protein of human CD8a. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300. Dilution: IH (1/100 - 1/200) Gene Symbol: CD8A Alternative Names: MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8aEntrez Gene (Human): 925SwissProt (Human): P01732Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Immunohistochemical analysis of CD8a staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.