Description: Recombinant rabbit monoclonal antibody to CD10 Immunogen: Recombinant fusion protein of human CD10. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MME Alternative Names: EPN; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10Entrez Gene (Human): 4311Entrez Gene (Mouse) : 17380Entrez Gene (Rat) : 24590SwissProt (Human): P08473SwissProt (Mouse) : Q61391SwissProt (Rat) : P07861Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD10 expression in mouse kidney (A), rat liver (B), rat kidney (C) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 85 kD)
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  Immunohistochemical analysis of CD10 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.