Description: Recombinant rabbit monoclonal antibody to Cytokeratin 5 Immunogen: Recombinant fusion protein of human Cytokeratin 5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: KRT5 Alternative Names: Keratin type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5Entrez Gene (Human): 3852Entrez Gene (Mouse) : 110308Entrez Gene (Rat) : 369017SwissProt (Human): P13647SwissProt (Mouse) : Q922U2SwissProt (Rat) : Q6P6Q2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytokeratin 5 expression in mouse kidney (A), rat kidney (B) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)
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  Immunohistochemical analysis of Cytokeratin 5 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.