Description: Rabbit polyclonal antibody to KLHL20 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human KLHL20. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: KLHL20 Alternative Names: KLEIP; Kelch-like protein 20; Kelch-like ECT2-interacting protein; Kelch-like protein XEntrez Gene (Human): 27252SwissProt (Human): Q9Y2M5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of KLHL20 expression in U251 (A), NCIH460 (B), K562 (C) whole cell lysates.
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Immunohistochemical analysis of KLHL20 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.