Description: Rabbit polyclonal antibody to GKAP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human GKAP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GKAP1 Alternative Names: GKAP42; G kinase-anchoring protein 1; cGMP-dependent protein kinase-anchoring protein of 42 kDaEntrez Gene (Human): 80318SwissProt (Human): Q5VSY0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GKAP1 expression in MDAMB435 (A) whole cell lysates.
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Immunohistochemical analysis of GKAP1 staining in human cervix carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.