Description: Rabbit polyclonal antibody to IPO7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human IPO7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: IPO7 Alternative Names: RANBP7; Importin-7; Imp7; Ran-binding protein 7; RanBP7Entrez Gene (Human): 10527Entrez Gene (Mouse) : 233726SwissProt (Human): O95373SwissProt (Mouse) : Q9EPL8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IPO7 expression in Hela (A) whole cell lysates.
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Immunohistochemical analysis of IPO7 staining in human cervix carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.