Description: Rabbit polyclonal antibody to IFT172 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human IFT172. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: IFT172 Alternative Names: KIAA1179; Intraflagellar transport protein 172 homologEntrez Gene (Human): 26160Entrez Gene (Mouse) : 67661SwissProt (Human): Q9UG01SwissProt (Mouse) : Q6VH22Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of IFT172 expression in mouse lung (A) whole cell lysates.
-
Flow cytometric analysis of 293 cells using Anti-IFT172 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.