Description: Rabbit polyclonal antibody to PAAF1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PAAF1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PAAF1 Alternative Names: WDR71; Proteasomal ATPase-associated factor 1; Protein G-16; WD repeat-containing protein 71Entrez Gene (Human): 80227SwissProt (Human): Q9BRP4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PAAF1 expression in A549 (A) whole cell lysates.
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Immunohistochemical analysis of PAAF1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.