Description: Rabbit polyclonal antibody to DDO Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human DDO. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: DDO Alternative Names: D-aspartate oxidase; DASOX; DDOEntrez Gene (Human): 8528Entrez Gene (Mouse) : 70503SwissProt (Human): Q99489SwissProt (Mouse) : Q922Z0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DDO expression in NCIH292 (A) whole cell lysates.
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Immunohistochemical analysis of DDO staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.