Description: Rabbit polyclonal antibody to PGAP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PGAP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/30) Gene Symbol: PGAP1 Alternative Names: GPI inositol-deacylase; Post-GPI attachment to proteins factor 1; hPGAP1Entrez Gene (Human): 80055Entrez Gene (Mouse) : 241062Entrez Gene (Rat) : 316400SwissProt (Human): Q75T13SwissProt (Mouse) : Q3UUQ7SwissProt (Rat) : Q765A7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PGAP1 expression in SHSY5Y (A), Hela (B), 293 (C), mouse kidney (D), mouse testis (E), rat kidney (F) whole cell lysates.
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Immunofluorescent analysis of Anti-PGAP1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
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Flow cytometric analysis of Hela cells using Anti-PGAP1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.