Anti-PGAP1 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of PGAP1 expression in SHSY5Y (A), Hela (B), 293 (C), mouse kidney (D), mouse testis (E), rat kidney (F) whole cell lysates.
Immunofluorescent analysis of Anti-PGAP1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-PGAP1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Product Name	Anti-PGAP1 Antibody
Cat No:	CQA9761
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IF/IC, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

50 μl

$150

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Description: Rabbit polyclonal antibody to PGAP1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PGAP1. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/30)

Gene Symbol: PGAP1

Alternative Names: GPI inositol-deacylase; Post-GPI attachment to proteins factor 1; hPGAP1

Entrez Gene (Human): 80055

Entrez Gene (Mouse) : 241062

Entrez Gene (Rat) : 316400

SwissProt (Human): Q75T13

SwissProt (Mouse) : Q3UUQ7

SwissProt (Rat) : Q765A7

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of PGAP1 expression in SHSY5Y (A), Hela (B), 293 (C), mouse kidney (D), mouse testis (E), rat kidney (F) whole cell lysates.
Immunofluorescent analysis of Anti-PGAP1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-PGAP1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

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