Description: Rabbit polyclonal antibody to PCSK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PCSK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/30) Gene Symbol: PCSK1 Alternative Names: NEC1; Neuroendocrine convertase 1; NEC 1; Prohormone convertase 1; Proprotein convertase 1; PC1Entrez Gene (Human): 5122Entrez Gene (Mouse) : 18548Entrez Gene (Rat) : 100911216;25204SwissProt (Human): P29120SwissProt (Mouse) : P63239SwissProt (Rat) : P28840Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PCSK1 expression in Hela (A), A549 (B), PC12 (C), mouse brain (D), NCIH460 (E) whole cell lysates.
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  Flow cytometric analysis of A549 cells using Anti-PCSK1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.