Description: Rabbit polyclonal antibody to USP47 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human USP47. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: USP47 Alternative Names: Ubiquitin carboxyl-terminal hydrolase 47; Deubiquitinating enzyme 47; Ubiquitin thioesterase 47; Ubiquitin-specific-processing protease 47Entrez Gene (Human): 55031SwissProt (Human): Q96K76Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of USP47 expression in Hela (A), human testis (B), U87MG (C) whole cell lysates.
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Immunohistochemical analysis of USP47 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.