Description: Rabbit polyclonal antibody to TSPEAR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human TSPEAR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50) Gene Symbol: TSPEAR Alternative Names: C21orf29; Thrombospondin-type laminin G domain and EAR repeat-containing protein; TSP-EAREntrez Gene (Human): 54084SwissProt (Human): Q8WU66Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TSPEAR expression in 293 (A) whole cell lysates.
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  Immunohistochemical analysis of TSPEAR staining in human lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Flow cytometric analysis of K562 cells using Anti-TSPEAR Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.