Description: Rabbit polyclonal antibody to TSPAN6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human TSPAN6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: TSPAN6 Alternative Names: TM4SF6; Tetraspanin-6; Tspan-6; A15 homolog; Putative NF-kappa-B-activating protein 321; T245 protein; Tetraspanin TM4-D; Transmembrane 4 superfamily member 6Entrez Gene (Human): 7105SwissProt (Human): O43657SwissProt (Mouse) : O70401Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of TSPAN6 expression in mouse bladder (A) whole cell lysates.
-
Flow cytometric analysis of Hela cells using Anti-TSPAN6 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.