Description: Rabbit polyclonal antibody to SPRR1B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human SPRR1B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SPRR1B Alternative Names: Cornifin-B; 14.9 kDa pancornulin; Small proline-rich protein IB; SPR-IBEntrez Gene (Human): 6699SwissProt (Human): P22528Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SPRR1B expression in MDAMB231 (A), MDAMB435 (B) whole cell lysates.
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Immunohistochemical analysis of SPRR1B staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.