Description: Rabbit polyclonal antibody to ANO5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human ANO5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ANO5 Alternative Names: GDD1; TMEM16E; Anoctamin-5; Gnathodiaphyseal dysplasia 1 protein; Transmembrane protein 16EEntrez Gene (Human): 203859Entrez Gene (Mouse) : 233246SwissProt (Human): Q75V66SwissProt (Mouse) : Q75UR0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ANO5 expression in human brain (A), U87MG (B) whole cell lysates.
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  Immunohistochemical analysis of ANO5 staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.