Description: Rabbit polyclonal antibody to SCAP Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human SCAP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/30) Gene Symbol: SCAP Alternative Names: KIAA0199; Sterol regulatory element-binding protein cleavage-activating protein; SCAP; SREBP cleavage-activating proteinEntrez Gene (Human): 22937Entrez Gene (Mouse) : 235623Entrez Gene (Rat) : 301024SwissProt (Human): Q12770SwissProt (Mouse) : Q6GQT6SwissProt (Rat) : A2RRU4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SCAP expression in Hela (A), HT29 (B), K562 (C), LNCap (D) whole cell lysates.
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Flow cytometric analysis of K562 cells using Anti-SCAP Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.