Description: Rabbit polyclonal antibody to PPM1F Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PPM1F. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PPM1F Alternative Names: KIAA0015; POPX2; Protein phosphatase 1F; Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog; hFem-2Entrez Gene (Human): 9647Entrez Gene (Mouse) : 68606SwissProt (Human): P49593SwissProt (Mouse) : Q8CGA0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PPM1F expression in mouse liver (A) whole cell lysates.
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Immunohistochemical analysis of PPM1F staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.