Description: Rabbit polyclonal antibody to WEE1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human WEE1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: WEE1 Alternative Names: Wee1-like protein kinase; WEE1hu; Wee1A kinaseEntrez Gene (Human): 7465SwissProt (Human): P30291Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of WEE1 expression in A431 (A) whole cell lysates.
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Immunohistochemical analysis of WEE1 staining in human hepatocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.