Description: Rabbit polyclonal antibody to MLK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human MLK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MAP3K9 Alternative Names: MLK1; PRKE1; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1Entrez Gene (Human): 4293SwissProt (Human): P80192Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MLK1 expression in Jurkat (A), SW480 (B) whole cell lysates.
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  Immunohistochemical analysis of MLK1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.