Description: Rabbit polyclonal antibody to ALOX5AP Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human ALOX5AP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ALOX5AP Alternative Names: FLAP; Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding proteinEntrez Gene (Human): 241SwissProt (Human): P20292Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ALOX5AP expression in K562 (A) whole cell lysates.
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Immunohistochemical analysis of ALOX5AP staining in human lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.