Description: Rabbit polyclonal antibody to Cytochrome P450 4A11 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Cytochrome P450 4A11. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50) Gene Symbol: CYP4A11 Alternative Names: CYP4A2; Cytochrome P450 4A11; 20-hydroxyeicosatetraenoic acid synthase; 20-HETE synthase; CYP4AII; CYPIVA11; Cytochrome P-450HK-omega; Cytochrome P450HL-omega; Fatty acid omega-hydroxylase; Lauric acid omega-hydroxylaseEntrez Gene (Human): 1579SwissProt (Human): Q02928Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytochrome P450 4A11 expression in NCIH460 (A) whole cell lysates.
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Immunofluorescent analysis of Anti-Cytochrome P450 4A11 staining in NCIH460 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).