Description: Rabbit polyclonal antibody to CARS2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CARS2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CARS2 Alternative Names: Probable cysteine--tRNA ligase, mitochondrial; Cysteinyl-tRNA synthetase; CysRSEntrez Gene (Human): 79587SwissProt (Human): Q9HA77Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CARS2 expression in NCIH460 (A) whole cell lysates.
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Immunohistochemical analysis of CARS2 staining in human hepatocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.